glucose kinase gene of Streptomyces coelicolor A3(2) and its role in glucose repression.

Glucose kinase gene of Streptomyces coelicolor A3(2) and its role in glucose repression by Susan Angell Download PDF EPUB FB2

Mutants (glk) of Streptomyces coelicolor A3(2) that are resistant to the non‐utilizable glucose analogue 2‐deoxyglucose are deficient in glucose kinase activity, defective in glucose repression, and usually unable to utilize glucose. The glucose kinase gene of Streptomyces coelicolor A3(2): its nucleotide sequence, transcriptional analysis and role in glucose repression.

Mol Microbiol. Oct; 6 (19)– Brawner ME, Auerbach JI, Fornwald JA, Rosenberg M, Taylor DP. Characterization of Streptomyces promoter sequences using the Escherichia coli galactokinase by: Glucose kinase of Streptomyces coelicolor A3(2) is essential for glucose utilisation and is required for carbon catabolite repression (CCR) exerted through glucose and other carbon sources.

The protein belongs to the ROK-family, which comprises bacterial sugar kinases and by:   A number of deletions in the glucose kinase (glk) region of the Streptomyces coelicolor chromosome were found among spontaneous glk mutants.

The deletions were identified by probing Southern blots of chromosomal DNA from glk mutants with cloned glk DNA. The deletions ranged in size from kb to greater than kb. When cloned glk DNA was introduced on a ϕC31 phage vector into Cited by: Glucose kinase of Streptomyces coelicolor A3(2) is essential for glucose utilisation and is required for carbon catabolite repression (CCR) exerted through glucose and other carbon sources.

The glucose kinase gene (glkA-ORF3) of Streptomyces coelicolor A3(2) plays an essential role in glucose utilisation and in glucose repression of a variety of genes involved in the utilisation of alternative carbon genes include dagA, which encodes an extracellular agarase that permits agar ssor mutants of glkA-ORF3 deletion strains capable of utilising glucose Author: Susan Angell.

The glucose kinase gene of Streptomyces coelicolor A3(2): its nucleotide sequence, transcriptional analysis and role in glucose repression. Mol Microbiol. Oct; 6 (19)– Angell S, Lewis CG, Buttner MJ, Bibb MJ.

Glucose repression in Streptomyces coelicolor A3(2): a likely regulatory role for glucose kinase. Mol Gen by: Short communication. A novel plasmid vector that uses the glucose kinase gene (glkA) for the positive selection of stable gene disruptants in Streptomyces☆. Abstract.

We describe an Escherichia coli plasmid, pIJ, that can be used for the efficient construction of stable gene disruptants and of gene deletions in by: Abstract. Using an antibiotic enrichment procedure, eight mutants of Streptomyces peucetius var.

caesius were isolated for their sensitivity to the glucose analogue 2-deoxyglucose (DOG), from a DOG-resistant strain (Dog R). These mutants (Dog S) and their parent strain were examined for growth sensitivity to DOG, glucose kinase (Glk) activity, Cited by: The glucose kinase gene (glkA-ORF3) of Streptomyces coelicolor A3(2) plays an essential role in glucose utilisation and in glucose repression of a variety of genes involved in the utilisation of.

In Streptomyces coelicolor A3(2), glucose kinase appears to play a regulatory role in carbon catabolite repression, and mutations in ccrA and in a variety of bld genes, result in the pleiotropic loss of glucose repression.

In none of these cases is the regulatory mechanism by: The glucose kinase gene of Streptomyces coelicolor A3(2): its nucleotide sequence, transcriptional analysis and role in glucose repression. Mol. Microbiol. 6: – We describe an Escherichia coli plasmid, pIJ, that can be used for the efficient construction of stable gene disruptants and of gene deletions in ation of pIJ derivatives carrying chromosomal sequences is achieved by selecting for plasmid-encoded thiostrepton resistance, while plasmid excision is secured by counter-selection of the pIJ glkA gene, which confers Cited by: Glucose kinase activity in the mutants reached about 75% of the wild-type level, indicating the presence of another enzyme in S.

xylosus. However, the cloned gene complemented an Escherichia coli strain in glucose kinase. Therefore, the glkA gene encodes a glucose kinase that participates in catabolite repression in S. by: Summary.

We provide a functional and regulatory analysis of glcP, encoding the major glucose transporter of Streptomyces coelicolor A3(2). GlcP, a member of the Major Facilitator Superfamily (MFS) of bacterial and eucaryotic sugar permeases, was found to be encoded twice at two distinct loci, glcP1 and glcP2, located in the central core and in the variable right arm of the chromosome.

Postma, ). As the mutations all map to the gene encoding glucose kinase and none to a transporter-encoding gene, it became obvious that more than one glucose permease must exist. The presence of a consti-tutive, low-affinity transport system has been reported for S. coelicolor and Streptomyces violaceoruber with affinityCited by: Mutants of Streptomyces coelicolor resistant to the non- utilizable glucose analogue, 2-deoxyglucose (DOG), appear to be generally deficient in glucose repression [9].

The lack of glucose repression in such mutants was correlated with the loss of ATP-dependent glucose kinase Cited by: Streptomyces coelicolor (strain ATCC BAA / A3(2) / M) Status.

Glucose kinase Gene names i: Name:glkA Alternatively, it may form a heterotetramer of two glucokinase subunits with two ORF2 (AC P) proteins. Protein-protein interaction databases STRING i: SCO Structure i 3D structure databases. CiteSeerX - Document Details (Isaac Councill, Lee Giles, Pradeep Teregowda): Glucose kinase of Streptomyces coelicolor A3(2) is essential for glucose utilisation and is required for carbon catabolite repression (CCR) exerted through glucose and other carbon sources.

The protein belongs to the ROK-family, which comprises bacterial sugar kinases and regulators. Streptomyces are well known for their production of biologically active secondary metabolites, with almost two-thirds of all known natural antibiotics being produced by best genetically characterized strain is Streptomyces coelicolor A3(2), which produces pigmented antibiotics and has become the preferred model organism in Streptomyces research.

A glucose kinase (glkA) mutant of Streptomyces coelicolor A3(2) M was selected by the ability to grow in the presence of the nonmetabolizable glucose analog 2-deoxyglucose.When a DNA fragment containing glkA, a gene for glucose kinase, of Streptomyces coelicolor A3(2) was introduced into strain G on a low-copy-number plasmid, the sensitivity to 2-DOG, the ability.The agarase gene (dagA) of Streptomyces coelicolor A3(2): nucleotide sequence and transcriptional analysis.

Mol Gen Genet. Aug; (1)– Buttner MJ, Smith AM, Bibb MJ. At least three different RNA polymerase holoenzymes direct transcription of the agarase gene (dagA) of Streptomyces coelicolor A3(2). by: